188 Section V ■ Vascular Access
with mosquito hemostat. C: Elevating artery with artery hook. D: Looping ligature around artery.
Chapter 31 ■ Peripheral Arterial Cannulation 189
6. Wait 5 minutes for anesthesia.
7. Make transverse incision (0.5 cm) posteroinferior to
medial malleolus (see Fig. 31.4).
A vertical, rather than a transverse, incision is
optional. The former has the advantage that it offers the
opportunity to extend the incision cephalad, should the
posterior wall of the vein be perforated on the initial
8. Identify artery by longitudinal dissection with mosquito
hemostat. The artery is usually found just anterior to
the Achilles tendon and adjacent to the tibial nerve.
9. Place mosquito hemostat behind artery, and loop 5-0
nylon suture loosely around it.
Be gentle, to avoid arteriospasm.
10. Elevate artery in wound with suture. Do not ligate
11. While stabilizing artery with suture, insert needle and
12. Withdraw stylet and advance cannula to hub.
14. Close wound with 5-0 nylon suture (usually requires
15. See percutaneous method under E (Standard Technique,
steps 7 to 11) for fixation and care of cannula.
3. Sterile 2- × 2-inch gauze squares (for three-drop method)
Surg. 1977;12:715, with permission.)
4. 25-gauge straight needle (for three-drop method)
5. Appropriate-sized syringe for sample (heparinized if
sample is not processed on site)
6. Syringe with flush (for stopcock method)
7. Ice if necessary for sample preservation
8. Specimen labels and requisition slips
Technique I: Three-Drop Method
1. Wash hands and put on gloves.
2. Clean diaphragm of T connector with antiseptic solution and allow to dry.
3. Clamp T-connector tubing close to hub.
4. Place sterile gauze squares beneath hub.
5. Introduce 25-gauge needle through diaphragm and
allow 3 to 4 drops of fluid/blood to drip onto gauze.
6. Attach syringe to needle and withdraw sample.
7. Remove needle from diaphragm.
8. Unclamp T connector and allow residual pump pressure to flush catheter.
Technique II: Stopcock Method (a Three-Way
Stopcock Needs to be Interposed between the
1. Wash hands and put on gloves.
2. Clean hub of stopcock with antiseptic solution.
3. Attach syringe to stopcock.
4. Turn stopcock off to infusion pump.
5. Aspirate waste (volume depends on length of tubing).
6. Using second syringe, withdraw sample.
7. Flush cannula slowly, over 30 to 60 seconds, with 0.5
8. Open stopcock to pump, to allow for continued infusion of heparinized saline.
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