Brian C. Kitamura, MD
John Sarka, MD
Key Points
• Arterial puncture for blood gas ana lysis is a common
procedure performed in the emergency department
(ED).
• Blood obtained from the radial artery can be used
to quickly provide qua ntitative information on the
INDICATIONS
The primary indication for obtaining an arterial blood
sample is for the assessment of the partial pressures of
oxygen and carbon dioxide and accurate assessment of
arterial pH. Secondarily, arterial blood can be analyzed
for carboxyhemoglobin, methemoglobin, and basic elec
trolytes depending on the capabilities of the laboratory.
Under certain circumstances it may be necessary to
obtain a sample of arterial blood for other routine laboratory tests, such as in patients who are obese or have a
history of intravenous drug abuse, in whom the radial
artery is palpable, but venous access is difficult or may be
delayed.
CONTRAINDICATIONS
There are few absolute contraindications for arterial puncture for blood gas analysis. Trauma, infection, or abnormalities of the overlying skin such as a burn are
contraindications because of concern for infection or further damage to the vascular structures. Patients with
known coagulopathies, taking anticoagulants, or who may
require thrombolytic agents should be approached with
caution because of the increased risk of bleeding, hema
toma formation, or rarely, compartment syndrome. Finally,
a known history of insufficient blood flow through the
4
patient's acid-base status and carboxyhemoglobin,
methemoglobin, and electrolyte levels.
• Arterial puncture is a useful way to obta in blood for
ana lysis when traditional phlebotomy is limited or difficult on the basis of patient characteristics.
palmar arch or previous surgery to the radial or ulnar
arteries should also be considered a contraindication. The
Allen test, described later, has been used as a way to determine adequacy of collateral circulation, however, its necessity has been questioned.
EQUIPMENT
Many commercially prepared kits for arterial puncture are
available, and if a commercial kit is not available, then
equipment is easily found in most EDs. The following
equipment is typically used to perform the procedure
(Figure 2-1).
� Required Equipment
Alcohol, chlorhexidine, or iodine prep pads
2- to 3-rnL heparinized syringe with a 23- to 25-gauge
needle
Syringe cap
Appropriate personal protective equipment
Gauze or other dressing
� Suggested Equipment
Anesthetic (eg, lidocaine)
Ultrasound or Doppler (if the artery is difficult to palpate)
ARTERIAL BLOOD GAS
Figure 2-1. Eq ui pment used for an arterial puncture.
Rolled towel or kidney basin (to stabilize and extend
the wrist)
Ice (for specimen process times > 10 minutes)
Local anesthesia is not strictly required for the procedure;
however, studies have shown that pain, as well as the
number of attempts required to obtain a sample, are
reduced when appropriate anesthesia is provided.
Traditionally, 1% lidocaine is used, avoiding epinephrine
because of concern for vasospasm. Recent studies have
suggested that jet-injected 2% lidocaine also provides
reasonable anesthesia.
PROCEDURE
Before selecting an appropriate wrist, the Allen test may
be used to assess collateral circulation. Manually occlude
the radial and ulnar arteries using your fingers. Ask the
patient to clench the fist to increase venous drainage
from the hand for approximately 30 seconds. Ask the
patient to open the hand, which should be noticeably
pale. At this point, release only the ulnar artery. Rapid
return of color signifies adequate collateral flow.
Although the necessity of the test for arterial puncture is
questioned, common sense dictates that if collateral flow
in one wrist is noticeably decreased compared with the
other, the wrist with better collateral flow should be
accessed. In the absence of good collateral flow in both
wrists, the necessity of the procedure should be weighed
against the remote risk of serious vascular injury and
distal extremity ischemia.
The radial artery is easily palpated in a majority of
patients. It runs down the radial aspect of the forearm,
generally located between the styloid process of the
radius and the flexor carpi radialis tendon at the
.A. Figure 2-2. Position of the forea rm for puncture of
the radial artery. A kidney basin or rol led towel may
be helpful to hold the patient's wrist in this position.
proximal crease of the wrist. The patient's wrist should
be extended to bring the artery to a more superficial
position. A kidney basin or rolled towel as well as tape
may be helpful to hold the patient's wrist in this posi
tion ( Figure 2-2). The skin overlying the artery should
be cleaned. The skin and immediate subcutaneous
tissue should then be appropriately anesthetized. The
authors recommend massaging the area or letting it rest
for 1-2 minutes for the anesthetic to take complete
effect. This time may be used to prepare your other
equipment.
After locating the impulse of the artery with the nondominate hand, take the syringe and needle in your dominate hand and slowly advance the needle toward the
impulse at a 30- to 45-degree angle proximally toward the
patient. If the impulse is difficult to detect, an ultrasound
or Doppler may be helpful to locate the artery (Figure 2 - 3) .
Some practitioners use a direct 90-degree angle to the skin,
but this is largely a matter of preference. When the artery
is accessed, blood will passively fill the syringe. It should
not be necessary to draw back on the syringe. Pulsatile or
bright red blood signals the correct vessel has been
accessed; however, this may not be apparent in the criti
cally ill patient. If blood is not obtained, withdraw the
needle to just below the skin and reattempt the procedure
after slight adjustments have been made. Do not move the
needle in an arc deep in the skin, as this risks damage to
the vascular structures.
After blood is collected, the needle should be removed
and disposed of appropriately. Remove air from the syringe
and place the syringe cap, ensuring that blood contacts the
cap. Maintain pressure over the arterial site for approxi
mately 5 minutes to prevent development of a hematoma,
and dress the wound appropriately.
CHAPTER 2
Radial vein
Tendon
Radial vein
8
Radial artery Tendon
Radial artery Tendon
A. Figure 2-3. The radial artery on ultrasonography. A. A high-frequency linear transducer is used to locate the vessel
based on anatomic landmarks. The ultrasound probe is oriented toward the patient's thumb. B. If there are other
vascu lar structures in the image, color Doppler can be used to locate the artery by identifyi ng pulsati le flow.
ARTERIAL BLOOD GAS
COMPLICATIONS
Complications from this procedure are rare but include
infection, bleeding, arterial laceration, pseudoaneurysm or
arteriovenous malformation, and nerve injury.
SUGGESTED READING
Dev SP, Hillmer MD, Ferri M. Arterial puncture for blood gas
analysis. N Engl J Med. 20 11; 364:e7.
Giner J, et al. Pain during arterial puncture. Chest. 1 996; 1 1 0:
1443-1445.
Hajiseyedjavady H, et al. Less painful arterial blood g as sampling
using jet injection of 2o/o lidocaine: a randomized controlled
clinical trial. Am ] Emerg Med. 20 1 2;30:1 1 00-1 104.
Haynes JM, Mitchell H. Ultrasound-guided arterial puncture.
Resp Care. 2010;55:1 754-1 756.
Shiver S, Blaivas M, Lyon M. A prospective comparison of
ultrasound-guided and blindly placed radial arterial cathe
ters. Acad Emerg Med. 2006;13:1 275-1279.
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