cover your mouth and nose with a damp cloth to filter
¾ Inform firefighting department of your area if you
feel the fire can go out of hand. Medium to large fires
should be reported irrespective of your preparedness to
Laboratory Equipment (Table 1.1)
¾ Use all laboratory equipment as per manufacturer’s
¾ Any instrument with moving parts, such as a centrifuge,
must be operated with a special regard for safety. Latch,
the lid before turning it on. On turning it off, do not
open the lid before it has come to a complete stop
¾ Autoclaves present special hazards. Strictly adhere to
manufacturer’s instruction to prevent explosions and
burns. Use insulated gloves while removing hot items
¾ Use glassware that is free of chips and cracks. Damaged
glassware is weakened and may break, resulting in
¾ Broken glass should be cleaned with a brush and
dustpan and not with bare hands
¾ Glass should not be discarded into regular trashcans,
but into rigid cardboard or plastic containers
¾ Wherever possible, replace glassware with plasticware.
¾ Hypodermic needles: Accidental inoculation, aerosol
¾ Centrifuges: Aerosols, splashing and tube breakages
¾ Culture stirrers, shakers, agitators: Aerosols, splashing
¾ Refrigeration: If flammable chemicals are stored within
them, the light switches, thermostats, etc. can provide
¾ Water baths: Provide ground for microorganismal
(The risk of acquiring hepatitis B from a needle stick is
30%, hepatitis C is 2 to 10% and HIV is 0.3%).
Equipment/Materials Employed to Eliminate/Reduce
¾ Laboratory apron: Assists in diminishing skin contacts
TABLE 1.1: Fire fighting equipment
Fire fighting material Used for Contraindicated for
Electrical fires, flammable liquids, a small
Water Paper, wood, fabric Electrical fires, flammable liquids, burning metal, alkali metal
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