Much of the contamination in the laboratory occurs as
a result of penetrating injuries caused by sharp objects and
the spilling and splashing of specimen materials.
2. Barrier precautions (mask, cap, plastic apron and
3. Careful handling of all kinds of sharps and needles.
6. Correct disposal of different kinds of wastes generated
Guidelines of Basic Practices and Procedures
¾ Prevention of puncture wounds, cuts and abrasions and
protection of existing wounds, skin lesions, conjunctiva
¾ Application of simple protective measures designed
to prevent contamination of the person and his/her
¾ Good basic hygiene practices, including regular
¾ Control of surface contamination by containment and
¾ Safe disposal of contaminated waste.
Biosafety Regulations for Laboratory Procedures
¾ Wear gloves when handling infectious materials or
where there is a possibility of exposure to blood and
other body fluids. All laboratories that work with
material that is potentially infected with HIV require a
generous supply of good quality gloves.
¾ Discard gloves whenever they are thought to have
become contaminated or perforated, wash your hands
and put on new gloves. Alternatively, where there are
economic constraints, wash gloved hands whenever
they get contaminated with blood/body fluids before
¾ Do not touch your eye, nose, or other exposed
membranes or skin with your gloved hands.
Sterilization (for Nondisposable Items)
¾ For sharps, reusable blades, cystoscopy instruments,
endoscopy instruments, use CIDEX (2% glutaraldehyde)
or 5% Korsolex. Disinfection usually occurs in
¾ Use autoclaving for other reusable items (e.g. needle
¾ Wherever, autoclaving is not possible, boiling must be
Divide waste into three parts at source.
i. Household type noninfectious waste:
• Place in puncture-proof container containing
disinfectant (1% bleach prepared every morning). Needles should ideally be burnt (machines
are available that operate on electricity)
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