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Diagnostic tympanocentesis is indicated in neonatal acute
otitis media (AOM) to target antibiotic therapy (1,2).
Tympanocentesis may be used for both diagnostic and
drainage purposes (3). The specific indications include
1. AOM not responding to antibiotics after 72 hours
2. AOM in severely immunocompromised infant
3. AOM in infant already on antibiotics
4. AOM with suppurative complications (e.g., mastoiditis,
5. To confirm the diagnosis when the clinical exam is not
1. Difficulty in confirming ossicular landmarks. One must
be able to identify the malleus and the annulus of the
tympanic membrane (TM) (Fig. 21.1).
2. Suggestion of abnormal anatomy. This is more likely in
patients with congenital malformation syndromes.
3. Suggestion of alternate pathology (e.g., cholesteatoma
2. Otoscope with open operating head and good light
3. Largest speculum that will fit the canal (2, 3, or 4 mm)
4. 18-gauge 3-inch spinal needle with 1-mL or 3-mL
6. 70% isopropyl alcohol in 3-mL syringe for cleaning and
7. Suction setup with 5-Fr Frazier ear suction
8. Culturettes with transport media
1. Patient safety and comfort require proper restraint, adequate light, and appropriate instruments.
2. The kindest way is to be quick, and this means having
3. Conscious sedation is feasible only if the child is stable
and has no issues with airway obstruction. It is not
needed past the point of puncturing the TM; usually no
4. Good visualization is paramount. Sufficient cleaning
must be done so that the malleus and the anterior
aspect of the annulus are clearly visible.
5. Avoid the posterior aspect of the tympanic membrane.
This is where the round window, stapes, and incus are
1. Restrain infant (see Chapter 4).
2. Position infant with the head turned so that the involved
ear is up. The assistant must keep the head still.
3. Rinse ear canal with alcohol solution from a 3-mL
syringe. This will provide antisepsis and initiate cleaning.
4. Let fluid run out or use suction.
5. Use otoscope to visualize canal and remove debris with
6. Align speculum to get best view of TM landmarks.
Pulling superiorly and laterally on the pinna will
improve visibility (Fig. 21.2).
7. Attach spinal needle to syringe, after bending it 45 to
60 degrees at the hub. This keeps the syringe out of
8. Hold needle at the hub and introduce it through the
otoscope. Puncture the drum anterior to the malleus at
or below the umbo level (Figs. 21.2 and 21.3).
9. Hold needle securely and have assistant draw back on
10. Place sample in appropriate transport medium.
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