1. Store the reagent at 2 to 8°C. Do not freeze. 2. The shelf-life of reagent is as per the expiry date mentioned on the reagent vial label.

 

SLIDE TEST FOR C-REACTIVE PROTEIN

(RHELAX CRP)

(Courtesy: Tulip Group of Companies)

Summary

The C-reactive protein (CRP) is a serum protein which is

synthesized in the liver. Its rate of synthesis and secretion

increases within hours of an acute injury or the onset

of inflammation and may reach as high as 20 times the

normal levels. Elevated serum concentration of CRP is an

unequivocal evidence of an active tissue damage process;

and CRP measurement, thus provides a simple screening test

for organic disorders. Apart from indicating inflammatory

disorders, CRP measurement helps in differential diagnosis,

in the management of neonatal septicemia and meningitis

where standard microbiological investigations are difficult.

Its use in postoperative surveillance is of great

importance. CRP levels invariably rise after major surgery

but fall to normal within 7–10 days. Absence of this fall is

indicative of possible septic or inflammatory postoperative

complications. Serum CRP measurement also provides

useful information in patients with myocardial infarction

there being an excellent correlation between peak levels of

CRP and creatine phosphokinase (CPK).

Reagent

1. RHELAX CRP reagent: A uniform suspension of

polystyrene latex particles coated with anti-CRP

antibodies. The reagent is standardized to detect CRP

concentrations greater than 0.6 mg/dl.

2. Positive control, reactive with RHELAX CRP reagent.

3. Negative control, non-reactive with RHELAX CRP

reagent.

Each batch of reagents undergoes rigorous quality

control at various stages of manufacture for its specificity,

sensitivity and performance.

Reagent Storage and Stability

1. Store the reagent at 2 to 8°C. Do not freeze.

2. The shelf-life of reagent is as per the expiry date

mentioned on the reagent vial label.

Principle

The RHELAX CRP slide test for detection of CRP is based

on the principle of agglutination. The test specimen

(serum) is mixed with RHELAX CRP latex reagent

and allowed to react. If CRP concentration is greater

than 0.6 mg/dl, a visible agglutination is observed.

If CRP concentration is less than 0.6 mg/dl, then no

agglutination is observed.