and/or monoclonal anti-P. vivax specific pLDH antibody and/or monoclonal pan specific pLDH antibody coated

 

Principle

Paramax-3 utilizes the principle of immunochromatography. As the test sample flows through the membrane

assembly of the device after addition of the clearing

buffer, the colored colloidal gold conjugates of antiHRP-2 antibody, anti-P. vivax specific pLDH antibody and

anti-pan specific pLDH antibody complexes the HRP-2/

corresponding pLDH in the lyzed sample. This complex

moves further on the membrane to the test region where it

is immobilized by the monoclonal anti-Pf. HRP-2 antibody

and/or monoclonal anti-P. vivax specific pLDH antibody

and/or monoclonal pan specific pLDH antibody coated

on the membrane leading to formation of a pink-purple

colored band in the respective regions which confirms

a positive test result. Absence of a colored band in the

test region indicates a negative test result for the corresponding antigen. The unreacted conjugate along with the

rabbit globulin colloidal gold conjugate and unbound

complex if any, move further on the membrane and are

subsequently immobilized by anti-rabbit antibodies coated

on the membrane at the control region, forming a pinkpurple band. This control band serves to validate the test

performance.