• Improper dispensing of the entire reagent from dropper
Immediately after performing the test, transfer the contents of the
reagent dropper back into the reagent vial
Ensure that no reagent is left behind in the dropper. Close the cap of
the reagent vial properly and store it back at 2–8°C
Do not freeze the reagent vial
1. The sensitivity of our kit is 0.6 mg/dL hence if another kit has a
Check the sensitivity of the another kit with known true CRP calibrator
before confirming the test results
Problem: Positive result with our kit and negative with another kit or vice versa
SLIDE TEST FOR ANTISTREPTOLYSIN O
(Courtesy: Tulip Group of Companies)
Streptococcus belongs to the family of Lactobacillaceae
and the majority are facultative anerobes. The facultative
anerobic streptococci are divided into two categories:
a.Those which produce soluble hemolysin and
b.Those which do not produce soluble hemolysin
The first group of streptococci are called β-hemolytic
streptococci which can be further subdivided into group
(a), group (b), group (c) and group (d). It includes most of the
species associated with primary streptococcal infections in
humans. The group (a) β-hemolytic streptococci produce
various exotoxins such as streptolysin O and streptolysin S
that can act as antigens. The affected individuals produce
specific antibodies against streptolysin O, namely anti
streptolysin O (ASO). Determination of these antibodies
is very useful for the diagnosis of streptococcal infections
and their relative effects such as rheumatic fever and acute
glomerulonephritis. An elevated ASO titer of more than
200 IU/mL may indicate an acute streptococcal infection.
1. RHELAX ASO reagent: A uniform suspension of
polystyrene latex particles coated with streptolysin
2. Positive control, reactive with RHELAX ASO reagent.
3. Negative control, nonreactive with RHELAX ASO
The RHELAX ASO reagent system is standardized to
detect antibodies to streptolysin O in concentrations
ranging from 200 to 4000 IU/mL.
Each batch of reagents undergoes rigorous quality
control at various stages of manufacture for its specificity,
1. Store the reagent at 2 to 8°C. Do not freeze.
2. The shelf-life of reagent is as per the expiry date
mentioned on the reagent vial labels.
The RHELAX ASO slide test for detection of antibodies to
streptolysin O is based on the principle of agglutination.
The test specimen (serum) is mixed with RHELAX ASO latex
reagent and allowed to react. If antibodies to streptolysin
O are present in concentrations more than 200 IU/mL,
but less than 4000 lU/mL, then a visible agglutination is
observed. If antibodies to streptolysin O are not present
or are in concentrations less than 200 IU/mL, then no
agglutination will be observed.
1. In vitro diagnostic reagent for laboratory and professional use only. Not for medicinal use.
2. All the reagents derived from human source have
been tested for HBsAg and anti-HIV antibodies and
are found to be non-reactive. However, handle the
3. The reagent contains sodium azide 0.1% as preservative.
Avoid contact with skin and mucosa. On disposal,
flush with large quantities of water.
4. The reagent can be damaged due to microbial
contamination or on exposure to extreme temperatures. It is recommended that the performance of
the reagent be verified with the positive and negative
controls provided with the kit.
5. Shake the RHELAX ASO latex reagent well before use
to disperse the latex particles uniformly and improve
6. Only a clean and dry glass slide must be used. Clean
the slide with distilled water and wipe dry.
7. Accessories provided with the kit only must be used
Specimen Collection and Preparation
No special preparation of the patient is required prior to
specimen collection by approved techniques.
Only serum should be used for testing. Should a delay
in testing occur, store the sample at 2 to 8°C. Samples can
be stored for up to a week. Do not use hemolyzed serum.
Material Provided with the Kit
Comments
Post a Comment
اكتب تعليق حول الموضوع