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85

Drug–Drug Interaction

PHARMACODYNAMIC—ADDITIVE EFFECTS OF MEDICATIONS

J.A.’s medications may also have additive effects with cisatracurium. A rare adverse

effect of amikacin is neuromuscular blockade. The mechanism of blockade involves

inhibiting acetylcholine release by competing with Ca

+2 at the preganglionic nerve

terminal and to a smaller degree noncompetitive blocking of the receptor.

86

Corticosteroids (e.g., hydrocortisone) may also enhance blockade and increase

recovery time. Proposed mechanisms for steroidal ICU-acquired weakness include

increased muscle sensitivity to corticosteroids because of lack of movement and

skeletal muscle atrophy from the steroid’s catabolic actions. Additionally,

corticosteroids may cause myopathy by denervation; corticosteroids have been

shown to inhibit the nicotinic receptor; when combined with the neuromuscular

blocking agent, vecuronium, this inhibition is potentiated.

76,87

It is thought that this

interaction is more likely to occur with neuromuscular blockers that have a steroid

structural ring, such as the aminosteroid (e.g., pancuronium, pipcuronium,

vecuronium, and rocuronium). However, there have been case reports of prolonged

paralysis with the benzylisoquinoliniums (e.g., atracurium, cisatracurium,

doxacurium, mivacurium, and d-tubocurarine).

78,79,88

J.A. may need a longer period than an hour and one-half to recover from his

paralysis because of the following factors: decreased elimination of cisatracurium as

a result of acidosis, hypophosphatemia, and medications (amikacin and

hydrocortisone). J.A. should also have his phosphate slowly repleted.

CASE 3-2, QUESTION 3: The medical team asks you to explain the drug interactions affecting the antibiotic

efficacy of J.A.’s regimen.

After reviewing the case, the clinician identifies potential drug–drug

physiochemical interaction, as well as drug–condition and drug–drug

pharmacodynamic interactions. The mechanism of action of these interactions is

discussed below.

Drug–Drug Interaction

PHYSIOCHEMICAL INACTIVATION

It has been well documented that the coadministration of beta-lactam antibiotics with

aminoglycoside antibiotics can lead to inactivation of the aminoglycoside. The

mechanism involves the amino group of the aminoglycoside antibiotic forming an

inactive amide with the beta-lactam ring of penicillin antibiotics.

89,90 Because

penicillins have wide therapeutic index, this interaction primarily affects the efficacy

of the aminoglycoside antibiotic.

p. 47

p. 48

This interaction has been shown to occur with the extended-spectrum penicillins

(e.g., azlocillin, carbenicillin, mezlocillin, ticarcillin, and piperacillin). J.A. is

currently on amikacin and imipenem–cilastatin antibiotics for treatment of a

multiresistant organism. According to the literature, amikacin is the aminoglycoside

that is least susceptible to this interaction.

90 Additionally, no inactivation of amikacin

was observed when incubated in cilastatin 120 µg/mL human serum for 48 h at

37°C.

91

This inactivation increases with contact time and is directly proportional to the

concentration of penicillin.

92 The rate of elimination of aminoglycoside and

imipenem–cilastatin may be increased because of J.A.’s renal dysfunction. This

would increase the contact time of the medications.

Recommendations for J.A.’s antibiotic therapy include administration of

medications separately; serum concentrations of aminoglycosides should be assayed

immediately after drawn or if analysis is delayed freeze at −70°C; and because of his

renal dysfunction, close monitoring of aminoglycoside serum concentrations is

indicated.

92,93

Drug-Condition/Disease Interaction

PHARMACODYNAMIC INTERACTION

The pharmacodynamic actions of amikacin may be decreased because J.A. is

acidotic.

Amikacin enters the bacterial cell and reaches its site of action in three stages:

ionic binding, energy-dependent phase I (EDP-I), and energy-dependent phase II

(EDP-II) transport or uptake.

Ionic Binding to the Outer Membrane: At physiologic pH, amikacin (pKa 8.1) is a

highly ionized basic cation. It binds to anionic lipopolysaccharides (LPSs), polar

heads of phospholipids, and proteins on the outer cell membrane of Gram-negative

bacteria and phospholipids and teichoic acids of Gram-positive bacteria.

94 This

leads to displacement of cell wall Mg

2+ and Ca

2+ bridges that link LPS, and the result

is the formation of pores in the cell wall where amikacin can enter into the

periplasmic space.

95

EDP-I: Amikacin is transported across the cytoplasmic membrane. EDP-I is

dependent on pH and oxygen. Amikacin activity will decline in low pH and

anaerobic conditions (e.g., abscesses).

95

EDP-II: Amikacin is transported to the site of action, binding to the ribosomes.

95

Drug–Drug Interaction

PHARMACODYNAMIC—ADDITIVE/SYNERGISTIC EFFECT OF

MEDICATIONS

Penicillins form a covalent bond with the enzymes, the penicillin-binding proteins

(PBPs) (specifically transpeptidase, endopeptidase, carboxypeptidase) inhibiting

their action. These enzymes are needed for the final step of bacterial cell wall

synthesis, the cross-linking between peptide side chains on the polysaccharide

backbones of the peptidoglycan.

96 Cell wall inhibitors such as penicillins and

vancomycin may expedite aminoglycoside entry into the bacterial cell resulting in

synergistic effects when treating some organisms.

95

J.A. is critically ill with renal failure, ARDS, pneumonia caused by a

multiresistant organism, septic shock, and a metabolic acidosis. It is important to

closely monitor his aminoglycoside therapy for efficacy (peaks) and toxicity

(troughs).

This case illustrates the difficulties surrounding drug interaction identification,

assessment, and follow-up intervention. Clinicians must recognize that literature to

support the presence of a drug interaction is often scant and not always definitive and

the optimal intervention may rely on clinical judgment. Refer to Chapter 56 for the

Care of the Critically Ill Adult Patient.

CASE 3-3

QUESTION 1: D.T. is a 67-year-old Caucasian male who began taking imatinib about 10 years ago to treat a

rare sarcoma: partially resected gastrointestinal stromal tumor (GIST). D.T. currently takes 600-mg imatinib

daily, as well as rabeprazole and furosemide. He states that he is currently not taking any nonprescription

medications. He continues to go to the cancer treatment center for continued monitoring. He contacts the

cancer clinic to let them know that in 4 weeks he will be traveling to Africa to go on a safari. He mentioned that

the friends that he will be traveling with told him that he will need malaria prophylaxis.

You are consulted regarding this request as the medical team wants to know whether there are any potential

drug interactions and which antimalarial agent would be an appropriate selection.

Imatinib mesylate belongs to a class of drugs known as selective tyrosine kinase

inhibitors (TKIs).

97

It inhibits the BCR-ABL tyrosine kinase, the constitutive

abnormal tyrosine kinase created by the Philadelphia chromosome abnormality in

chronic myeloid proteins.

97

It also inhibits the tyrosine kinase for platelet-derived

growth factor (PDGF) and c-kit. TKIs, such as imatinib, are extensively metabolized

via cytochrome P450 enzymes (with a large degree of interindividual variability).

98

Imatinib is metabolized primarily by CYP 3A4, whereas CYP1A2, CYP2C9,

CYP2C19, CYP2D6, and CYP3A5 are reported to have a minor role in its

metabolism.

99

In addition, imatinib is a substrate of human organic cation transporter

type 1 (hOCT1), Pgp, and BCRP, though it is unclear whether imatinib is a substrate

or inhibitor of BCRP.

100–103

Imatinib also competitively inhibits the metabolism of

drugs that are CYP2C9, CYP2C19, CYP2D6, and CYP3A4 substrates.

104

It is also

highly protein bound with approximately 95% bound to human plasma

proteins.

99,105–107

Drug–Drug Interaction

PHARMACOKINETICS—DRUG METABOLISM/ELIMINATION

There are several considerations of potential drug interactions with imatinib. Drug

interactions should be considered when imatinib is administrated with other agents in

the CYP3A family.

97

In particular, interactions are likely with inhibitors of CYP3A4,

such as voriconazole or amiodarone, resulting in increases in the plasma

concentration of imatinib. Concomitant use of rifampicin or other strong CYP3A4

inducers with imatinib should be avoided. In addition, concomitant administration of

imatinib with agents that are both inhibitors of CYP3A4 and P-gp increases plasma

and intracellular imatinib concentrations. Examples of dual CYP3A4 and Pgp

inhibitors include verapamil, erythromycin, clarithromycin, ketoconazole,

fluconazole, and itraconazole.

100,108,109 TKIs, such as imatinib, also can inhibit drug

transporters and enzymes, resulting in changes in the exposure of coadministered

drugs. St. John’s-wort significantly altered the pharmacokinetic profile of imatinib

with reductions of 30% in the medium area under the concentration–time curve

(AUC). Patients should be cautioned regarding the concomitant use of products, such

as St. John’s-wort, as well as other inducers, that may necessitate an increase in

imatinib dosing to maintain therapeutic efficacy.

110,111 Drug interactions involving

protein binding of imatinib and other highly protein-bound drugs are not well

understood.

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