This test is based on the development of colors ranging
from buff-pink, for a negative reading, to purple when
acetoacetic acid reacts with nitroprusside.
This test is based on the apparent pKa change of
certain pretreated polyelectrolytes in relation to ionic
concentration. In the presence of an indicator, colors range
from deep blue-green in urine of low ionic concentration
through green and yellow-green in urines of increasing
This test is based on the peroxidase-like activity of hemoglobin,
color ranges from orange through green; very high levels
of blood may cause the color development to continue to
The test is based on the double indicator principle that
gives a broad range of color covering the entire urinary pH
range. Colors range from orange through yellow and green
This test is based on the protein-error-of-indicators
principle. At a constant pH, the development of any green
color is due to the presence of protein. Colors range from
yellow for “Negative” through yellow-green and green to
green-blue for “Positive” reactions.
This test is based on a modified Ehrlich reaction, in which
ρ-diethylaminobenzaldehyde in conjunction with a color
enhancer reacts with urobilinogen in a strongly acid
medium to produce a pink-red color.
(Based on dry weight at time of impregnation):
2.2% w/w glucose oxidase (microbial, 1.3 IU); 1.0% w/w
peroxidase (horseradish, 3300 IU); 8.1% w/w potassium
iodide; 69.8% w/w buffer; 18.9% w/w non-reactive
0.4% w/w 2, 4-dichloroaniline diazonium salt; 37.3% w/w
buffer; 62.3% w/w non-reactive ingredients.
7.1% w/w sodium nitroprusside; 92.9% w/w buffer.
2.8% w/w bromothymol blue; 68.8% w/w poly (methyl vinyl
ether/maleic anhydride); 28.4% w/w sodium hydroxide.
6.8% w/w disopropylbenzene dihydroperoxide; 4.0% w/w
3.3’, 5, 5’-tetramethylbenzidine; 48.0% w/w buffer; 41.2%
0.2% w/w methyl red; 2.8% w/w bromothymol blue; 97.0%
0.3% w/w tetrabromophenol blue; 97.3% w/w buffer; 2.4$
0.2% w/w ρ-diethylaminobenzaldehyde; 99.8% w/w nonreactive ingredients.
Bayer reagent strips are for in vitro diagnostic use.
Storage below 30°C in a cool, dry place. Do not refrigerate.
Keep out of direct sunlight. Do not use after expiration
Recommended Procedures for Handling
All unused strips must remain in the original bottle.
Transfer to any other container may cause reagent strips
to deteriorate and become unreactive. Do not remove
desiccant (s) from bottle. Do not remove strip from
the bottle until immediately before it is to be used
for testing. Replace cap immediately and tightly after
removing reagent strip. Do not touch test areas of the
reagent strip. Work areas and specimen containers
should be free of detergents and other contaminating
Dip test areas in urine completely, but briefly, to avoid
dissolving out the reagent. If using strips visually, read
test results carefully at the time specified, in a good light
(such as fluorescent) and with the test area held near the
appropriate color chart on the bottle label. Do not read
appropriate instrument-operating manual.
Protection against ambient moisture, light and heat is essential
to guard against altered reagent reactivity. Discoloration
or darkening of reagent areas may indicate deterioration.
If this is evident, or if test results are questionable or
inconsistent with expected findings, the following steps are
1. Confirm that the product is within the expiration date
2. Check performance against known negative and
positive control materials (e.g. Chek-stix Control
3. Retest with fresh product. If proper results are not
obtained, consult your local product representative
for advice on testing technique and results.
Specimen Collection and Preparation
Collect urine in a clean container and test it as soon as
possible. Do not centrifuge. The use of urine preservatives
is not recommended. If testing cannot be done within an
hour after voiding, refrigerate the specimen immediately
and let it return to room temperature before testing.
It is especially important to use fresh urine to obtain
optimal results with the tests for bilirubin and urobilinogen,
as these compounds are very unstable when exposed to
Prolonged exposure of urine to room temperature may
result in microbial proliferation with resultant changes in
pH. A shift to alkaline pH may cause false positive results
with the protein test area. Urine containing glucose may
decrease in pH as organisms metabolize the glucose.
Bacterial growth from contaminating organisms may
cause false positive blood reactions from the peroxidases
Contamination of the urine specimen with skin
cleansers containing chlorhexidine may affect protein
(and to a lesser extent specific gravity and bilirubin) test
results. The user should determine whether the use of such
Must be followed exactly to achieve reliable test results:
1. Collect fresh urine specimen in a clean, dry container.
Mix well immediately before testing.
2. Remove one strip from bottle and replace cap.
Completely immerse reagent areas of the strip in fresh
urine and remove immediately to avoid dissolving out
3. While removing, run the edge of the entire length of the
strip against the rim of the urine container to remove
excess urine. Hold the strip in a horizontal position to
prevent possible mixing of chemicals from adjacent
reagent areas and/or contaminating the hands with
times specified. Hold strip close to color blocks
b. If reading instrumentally, carefully follow the
directions given in the appropriate instrumentoperating manual.
Proper read time is critical for optimal results. If using
strips visually, read the glucose and bilirubin tests at 30
seconds after dipping. Read the ketone test at 40 seconds;
the specific gravity test at 45 seconds; pH, protein,
urobilinogen and blood at 60 seconds. The pH and protein
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