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of both, solute and solvent add up to 100, regardless of the final volume produced. A 25% solution would be 25 grams of solute dissolved in 75 grams of solvent. These solutions TABLE 4.1: Commonly employed indicators

 


TABLE 3.4: Enzyme

Contd...

Conventional unit IU/L Equivalent

Acid phosphatase (prostatic)

Bodansky 5.37

Shinowara-Jones-Reinhart 5.37

King-Armstrong 1.77

Bessey-Lowry-Brock 16.67

Alkaline phosphatase

Bodansky 5.37

Shinowara-Jones-Reinhart 5.37

King-Armstrong 7.1

Bessey-Lowry-Brock 16.67 0.14

Babson 1.0

SI Units 49

Contd...

TABLE 3.5: Therapeutic and toxic drugs

Contd...

 Conversion factors

Analyte

Conventional

units

SI units Conventional to SI units SI to conventional

units

Acetaminophen µg/mL µmol/L 6.62 0.151

Amikacin µg/mL µmol/L 1.71 0.585

Amitriptyline ng/mL nmol/L 3.61 0.277

Amobarbital µg/mL µmol/L 4.42 0.226

Amphetamine ng/mL

µg/mL

nmol/L

µmol/L

7.4

7.4

0.135

0.135

Bromide µg/mL mmol/L 0.0125 79.9

Caffeine µg/mL µmol/L 5.15 0.194

Carbamazepine (Tegretol) µg/mL µmol/L 4.23 0.236

Carbenicillin µg/mL µmol/L 2.64 0.378

Chloral hydrate µg/mL µmol/L 6.69 0.149

Chloramphenicol µg/mL µmol/L 3.09 0.323

Chlordiazepoxide (Librium) ng/mL µmol/L 0.00334 300

Chlorpromazine (Thorazine) ng/mL nmol/L 3.14 0.319

Chlorpropamide (Diabinese) µg/mL µmol/L 3.61 0.227

Conventional unit IU/L Equivalent

Aldolase

Sibley-Lehninger 0.74

Amylase

Somogyi (saccharogenic) 1.85

Somogyi 20.6 0.541

Creatine kinase (CK) 1.0

Hydroxybutyric dehydrogenase (d-HBD)

Rosalki-Wilkinson 0.482

Isocitrate dehydrogenase (ICD)

Wolfson-Williams-Ashman 0.0167

Taylor-Friedman 0.0167

Lactate dehydrogenase (LDH)

Wroblewski 0.482

Lipase

Cherry-Crandal 278

Malic dehydrogenase (MD)

Wacker-Ulmer-Valee 0.482

Transaminases

Reitman-Frankel 0.482

Karmen 0.482

50 Concise Book of Medical Laboratory Technology: Methods and Interpretations Contd...

Contd...

 Conversion factors

Analyte

Conventional

units

SI units Conventional to SI units SI to conventional

units

Cimetidine (Tagamet) µg/mL µmol/L 3.96 0.252

Clonazepam (Klonopin) ng/mL nmol/L 3.17 0.316

Clonidine (Catapres) ng/mL nmol/L 4.35 0.230

Cocaine ng/mL nmol/L 3.3 0.303

Codeine ng/mL nmol/L 3.34 0.299

Demerol (Meperidine) ng/mL nmol/L 4.04 0.247

Desipramine (Norpramin) ng/mL nmol/L 3.75 0.267

Diazepam (Valium) ng/mL µmol/L 0.0035 285

Digitoxin ng/mL nmol/L 1.31 0.765

Digoxin ng/mL nmol/L 1.28 0.781

Dilaudid ng/mL nmol/L 4.85 0.206

Disulfiram µg/mL µmol/L 12.12 0.0761

Doxepin (Sinequan) ng/mL nmol/L 3.58 0.279

Ethanol mg/dL mmol/L 0.217 4.61

Ethchlorvynol (Placidyl) µg/mL µmol/L 6.92 0.145

Ethosuximide (Zarontin) µg/mL µmol/L 7.08 0.141

Gentamicin µg/mL µmol/L 2.09 0.478

Glutethimide (Doriden) µg/mL µmol/L 4.60 0.217

Haloperidol (Haldol) ng/mL nmol/L 2.66 0.376

Ibuprofen µg/mL µmol/L 4.85 0.206

Imipramine (Tofranil) ng/mL nmol/L 3.57 0 28

Isoniazid µg/mL µmol/L 7.29 0.137

Kanamycin (Kantrex) µg/mL µmol/L 2.06 0.485

Lidocaine (Xylocaine) µg/mL µmol/L 4.27 0.234

Lithium mEq/L mmol/L 1 l

Lorazepam ng/mL nmol/L 3.11 0.321

LSD (lysergic acid diethylamide) µg/mL µmol/L 3.09 0.323

Meprobamate mg/L µmol/L 4.58 0.218

Methadone ng/mL µmol/L 0.00323 309

Methaqualone (Quaalude) µg/mL µmol/L 4.0 0.250

Methotrexate ng/mL nmol/L 2.2 0.454

Methsuximide µg/mL µmol/L 5.29 0.189

Methyldopa (Aldomet) µg/mL µmol/L 4.73 0.211

Morphine ng/mL

ng/mL

nmol/L

µmol/L

3.5

0.0035

0.285

285

Nortriptyline ng/mL nmol/L 3.8 0.263

Oxazepam µg/mL µmol/L 3.49 0.287

Paraldehyde µg/mL µmol/L 7.57 0.132

SI Units 51

Contd...

 Conversion factors

Analyte

Conventional

units

SI units Conventional to SI units SI to conventional

units

Pentobarbital (Nembutal) µg/mL µmol/L 4.42 0.179

Percodan ng/mL nmol/L 3.17 0.315

Phenacetin µg/mL µmol/L 5.58 0.179

Phenobarbital (Luminal) µg/mL µmol/L 4.31 0.232

Phenylbutazone (Butazolidin) µg/mL µmol/L 3.08 0.324

Phenytoin (Dilantin) µg/mL µmol/L 3.96 0.253

Primidone µg/mL µmol/L 4.58 0.218

Procainamide (Pronestyl), procaine (Novacain) µg/mL µmol/L 4.23 0.236

Propoxyphene (Darvon) µg/mL µmol/L 3.07 0.326

Propranolol ng/mL nmol/L 3.86 0.259

Quinidine µg/mL µmol/L 3.08 0.324

Quinine µg/mL µmol/L 3.08 0.324

Salicylic acid µg/mL µmol/L 7.24 0.138

Secobarbital (Seconal) µg/mL µmol/L 4.2 0.238

Theophylline (Aminophylline) µg/mL µmol/L 5.55 0.180

Tobramycin µg/mL µmol/L 2.14 0.467

Valproic acid µg/mL µmol/L 6.93 0.144

Vancomycin µg/mL mg/L 1 1

Warfarin (Coumadin) µg/mL µmol/L 3.24 0.308

INDICATORS

Indicators are usually acids of weak strength whose molecules

in solution are of a different color than their anions.

(pH: Neutral pH = 7.0, less than 7.0 is acidic, more than 7.0

is alkaline).

The color of an indicator solution depends on the

degree of dissociation of the indicator, and on the pH of

the solution. Supposing the weak acid indicator is H Indic’,

it would dissociate as:

H Indic’ H+ + + Indic—

(color-X) (hydrogen ion) (Color Y)

All acids contain hydrogen ions, so addition of an acid

would make the reaction shift from right to left (change

of color from Y to X) and addition of an alkali (alkalies

contain OH–

 or hydroxyl ions) would lead to production of

water (H2O→H++ OH–

) as the OH–

 radicals will associate

with and remove H+ ions causing a shift to the right of

the reaction (change of color from X to Y). Indicators that

just show whether a solution is an acid or alkali are called

broad indicators while some indicators change color at a

precise pH.

The list of commonly employed indicators are given in

Table 4.1.

SOLUTES, SOLVENTS AND SOLUTIONS

Solute

Solute is any substance that dissolves in a liquid.

Solvent

Solvent is any liquid in which a solute dissolves.

Solution

A solvent becomes a solution after dissolving a solute.

Buffer Solution

At any given temperature, these solutions retain their definite

pH and maintain it even after adding considerable amounts

of acids or alkalies. These solutions generally consist of a

weak acid mixed with its sodium or potassium salt.

Strength of a Solution

Solution strength can be expressed in four ways:

(1) Percent solutions, (2) Part dilutions, (3) Molar solutions,

and (4) Normal solutions.

Percent Solutions

This is the most usual way of expressing solution strength.

Percent implies per hundred and a 30% solution of

anything should contain 30 parts of solute per hundred

parts of the final solution.

Percent solution can further be expressed in three ways.

Weight per unit weight (w/w). This implies that the weight

of both, solute and solvent add up to 100, regardless of the

final volume produced. A 25% solution would be 25 grams

of solute dissolved in 75 grams of solvent. These solutions

TABLE 4.1: Commonly employed indicators

Indicator Color in acid

solution

Color in alkaline

solution

pH

ranges

Litmus Red Yellow 5.5–8.0

Phenophthalein Colorless Red 8.0–9.8

Methyl red Red Yellow 4.2–6.3

Methyl orange Red Orange 3.1–4.4

Phenol red Yellow Blue 6.8–8.4

Bromothymol blue Yellow Blue 6.0–7.6

4

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