Anti-H. pylori IgG Immunoenzymometric/Sandwich

(Streptavidin Biotin)

Anti-H. pylori IgM Immunoenzymometric/Sandwich

(Streptavidin Biotin)

Anti-H. pylori IgA Immunoenzymometric/Sandwich

(Streptavidin Biotin)

Human growth Immunoenzymometric/Sandwich

hormone (GH) (Streptavidin Biotin)

Cortisol Immunoenzymometric/Sandwich

(Streptavidin Biotin)

cTroponin-I Immunoenzymometric/Sandwich

(Streptavidin Biotin)

Myoglobin Immunoenzymometric/Sandwich

(Streptavidin Biotin)

CK-MB Immunoenzymometric/Sandwich

(Streptavidin Biotin)

hs CRP Immunoenzymometric/Sandwich

(Streptavidin Biotin)

Digoxin Competitive

Neonatal TSH Immunoenzymometric/Sandwich

(Streptavidin Biotin)

Neonatal T4 Competitive

Thyroglobulin Immunoenzymometric/Sandwich

(Streptavidin Biotin)

Parameter Principle

HAV Ab Competitive ELISA

HAV Ab Ig M Mu-Capture

HBeAg Sandwich ELISA

HBeAb Competitive ELISA

HbeAg/Ab

HBsAb Quantitative indirect ELISA

HBcAb Competitive ELISA

HBclgM Mu-Capture

HBsAg One step Sandwich ELISA

HCV Ab 4th generation, indirect ELISA

HCV IgM Indirect ELISA

HDV Ab Competitive ELISA

HDV IgG Indirect ELISA

HDV IgM Mu-Capture

HEV Ab

HEV IgG

HEV IgM

HIV Indirect ELISA 3rd gen

TB IgG Indirect ELISA

TB IgA Indirect ELISA

TB IgM Indirect ELISA

Toxo avidity Avidity, Indirect ELISA

Toxo IgG Interference corrected

Toxo IgM (Mu) Mu-Capture

Toxo IgM (IC) Interference corrected

Rubella IgG Interference corrected

Rubella IgM (Mu) Mu-Capture

Rubella IgM (IC) Interference corrected

Rubella avidity Indirect ELISA

CMV IgG Interference corrected

CMV IgM (Mu) Mu-Capture

CMV IgM (IC) Interference corrected

CMV avidity Avidity, indirect ELISA

HSV 1+2 IgG Interference corrected

HSV 1+2 IgM Interference corrected

HSV 1 IgG Interference corrected

HSV 1 IgM Interference corrected

HSV 2 IgG Interference corrected

HSV2 IgM Interference corrected

TORCH screen IgG Interference corrected

TORCH screen IgM Interference corrected

TORCH CHEMI

Toxo IgG Interference corrected

Toxo IgM (Mu) Mu-Capture

Rubella IgG Interference corrected

Rubella IgM (Mu) Mu-Capture

CMV IgG Interference corrected

CMV IgM (IC) Interference corrected

HSV 1+2 IgG Interference corrected

HSV 1+2 IgM Interference corrected

TORCH screen IgG

TORCH screen IgM

Testosterone Competitive ELISA

Androstenedione Competitive ELISA

DHEA-S (Dehydroepi- Competitive ELISA

androsterone sulfate)

Estriol-Free Competitive ELISA

Estriol-Total Competitive ELISA

17- Estodiol Competitive ELISA

17-OH Progestirol Competitive ELISA

Progesterone Competitive ELISA

Cortisol Competitive ELISA

Free Testosterone Competitive ELISA

TORCH parameters available in ELISA and CLIA formats

Parameter Principle

Rheumatology

Total ANA screen Indirect ELISA/Sandwich ELISA

Anti-ds DNA screen Indirect ELISA/Sandwich ELISA

ANA Combi ELISA Indirect ELISA/Sandwich ELISA

(8 Antigen)

EIA Anti-SS-A Indirect ELISA/Sandwich ELISA

EIA Anti-SS-B Indirect ELISA/Sandwich ELISA

EIA Anti-Sm Indirect ELISA/Sandwich ELISA

EIA Anti-Sm/RNP Indirect ELISA/Sandwich ELISA

EIA Anti-Scl-70 Indirect ELISA/Sandwich ELISA

EIA Anti-Jo-1 Indirect ELISA/Sandwich ELISA

RNP - 70 Indirect ELISA/Sandwich ELISA

EIA Anti-ssDNA Indirect ELISA/Sandwich ELISA

ENA screen Indirect ELISA/Sandwich ELISA

ENA combi Indirect ELISA/Sandwich ELISA

ENA-4-profile Indirect ELISA/Sandwich ELISA

ENA-6-profile Indirect ELISA/Sandwich ELISA

Centomere B Indirect ELISA/Sandwich ELISA

Anti-histone antibody Indirect ELISA/Sandwich ELISA

Anti-nucleosome Indirect ELISA/Sandwich ELISA

antibody

Anti-alpha fodrin Indirect ELISA/Sandwich ELISA

antibody

Contd...

Contd...

Contd...

Contd...

Serology/Immunology 597

DNase activity Indirect ELISA/Sandwich ELISA

Anti-C1q Indirect ELISA/Sandwich ELISA

Anti-Mutated Indirect ELISA/Sandwich ELISA

Citrullinated vimentin

(MCV)

Anti-Rib-P Indirect ELISA/Sandwich ELISA

SSA 52 Indirect ELISA/Sandwich ELISA

SSA 60 Indirect ELISA/Sandwich ELISA

Rheumatoid factor Indirect ELISA/Sandwich ELISA

screen

Rheumatoid factor Indirect ELISA/Sandwich ELISA

IgG

Rheumatoid factor Indirect ELISA/Sandwich ELISA

IgM

Rheumatoid factor Indirect ELISA/Sandwich ELISA

IgA

DNase activity Direct ELISA/Sandwich ELISA

Vasculitis

C-ANCA (PR 3) Indirect ELISA/Sandwich ELISA

ANCA COMBI (P+C) Indirect ELISA/Sandwich ELISA

GBM Indirect ELISA/Sandwich ELISA

Anti-BPI Indirect ELISA/Sandwich ELISA

Anti-Elastase Indirect ELISA/Sandwich ELISA

Anti-Cathepsin G Indirect ELISA/Sandwich ELISA

Anti-Lysozyme Indirect ELISA/Sandwich ELISA

Anti-Lactoferrin Indirect ELISA/Sandwich ELISA

ANCA Screen

Thrombosis

Anti-Phospholipid Indirect ELISA/Sandwich ELISA

Screen

Phosphatidyl serine Indirect ELISA/Sandwich ELISA

Anti-Cardiolipin IgA Indirect ELISA/Sandwich ELISA

Anti-Cardiolipin Indirect ELISA/Sandwich ELISA

Screen (IgG/IgM /IgA)

Anti-β2-Glycoprotein Indirect ELISA/Sandwich ELISA

I IgG/IgM

Anti-β2-Glycoprotein I Indirect ELISA/Sandwich ELISA

IgA

Anti-β2-Glycoprotein I Indirect ELISA/Sandwich ELISA

Screen

Anti-Prothrombin Indirect ELISA/Sandwich ELISA

IgG/IgM

Anti-Prothrombin IgA Indirect ELISA/Sandwich ELISA

Anti-Prothrombin Indirect ELISA/Sandwich ELISA

Screen

Anti-Annexin V Indirect ELISA/Sandwich ELISA

Anti-Phospholipid Indirect ELISA/Sandwich ELISA

Screen IgG/IgM

Anti-Phosphatidyl Indirect ELISA/Sandwich ELISA

Inositol IgG/IgM

Anti-Phosphatidic Indirect ELISA/Sandwich ELISA

Acid IgG/IgM

Thyroid

Thyroglobulin (Tg) Indirect ELISA/Sandwich ELISA

Anti-thyroperoxidase Indirect ELISA/Sandwich ELISA

(TPO)

Anti-thyroglobulin Indirect ELISA/Sandwich ELISA

Gastrointestinal

Anti-Parietal cell Indirect ELISA/Sandwich ELISA

Anti-Gliadin IgG Indirect ELISA/Sandwich ELISA

Anti-Gliadin IgA Indirect ELISA/Sandwich ELISA

Anti-Gliadin screen Indirect ELISA/Sandwich ELISA

Anti-Tissue transglu- Indirect ELISA/Sandwich ELISA

taminase IgA

Anti-Tissue transglu- Indirect ELISA/Sandwich ELISA

taminase IgG

Anti-Tissue transglu- Indirect ELISA/Sandwich ELISA

taminase screen

Anti-Mitochondrial Indirect ELISA/Sandwich ELISA

Antibody-M2

Anti-Saccharomyces Indirect ELISA/Sandwich ELISA

cerevisiae antibody

(ASCA)

Anti-Intrinsic factor Indirect ELISA/Sandwich ELISA

Diabetes

Anti-Insulin Indirect ELISA/Sandwich ELISA

Miscellaneous

Ferritin Indirect ELISA/Sandwich ELISA

Beta-2-microglobulin Indirect ELISA/Sandwich ELISA

Microalbumin Competitiive ELISA

Immunoblots

Gastro-5-Line Nitrocellulose membrane based

indirect immunoassay

ANA-9-Line

Nucleo-9-Line

EXAMPLES OF DETAILED ELISA METHODS

Competitive ELISA

Total Triiodothyronine (tT3)

(Courtesy: Lilac Medicare)

Intended Use: The quantitative determination of total

triiodothyronine concentration in human serum or

plasma by a microplate enzyme immunoassay.

Mfd: Monobind Inc.

Principle

Competitive Enzyme Immunoassay

The essential reagents required for a solid phase enzyme

immunoassay include immobilized antibody, enzymeantigen conjugate and native antigen.

Upon mixing immobilized antibody, enzyme-antigen

conjugate and a serum containing the native antigen, a

Contd...

Contd...

Contd...

598 Concise Book of Medical Laboratory Technology: Methods and Interpretations competition reaction results between the native antigen

and the enzyme-antigen conjugate for a limited number of

insolubulized binding sites. The interaction is illustrated

by the following equation:

 ka

EnzAg + Ag + AbC.W. AgAbC.W. + EnzAgAbC.W.

 k-a

 AbC.W. = Monospecific immobilized

Antibody (constant quantity)

     Ag = Native antigen (variable quantity)

 EnzAg = Enzyme-antigen conjugate

(constant quantity)

 AgAbC.W. = Antigen-antibody complex

EnzAg AbC.W. = Enzyme-antigen conjugate -

antibody complex

 ka = Rate constant of association

 k-a = Rate constant of dissociation

 K = ka/k-a = Equilibrium constant

After equilibrium is attained, the antibody-bound

fraction is separated from unbound antigen by

decantation or aspiration. The enzyme activity in the

antibody-bound fraction is inversely proportional to the

native antigen concentration. By utilizing several different