Note

To avoid glycolysis the serum should be separated

from the clot as soon as possible, and plasma should be

collected in an EDTA + fluoride bulb (0.5 mg + 1 mg per

ml of blood).

System Parameters

Reaction : End point Interval :

Wavelength : 505 nm Sample

volume

: 0.01 mL

Zero setting : Reagent blank Reagent

volume

: 1.00 mL

Incubation

temperature

: 37°C/RT Standard : 100 mg/dL

Incubated

time

: 10 min/30 min Factor :

Delay time : — React slope : Increasing

Read time : — Linearity : 500 mg/dL

No. of read : — Units : mg/dL

Body Fluid, Glucose

Normal Values

SI units

Cerebrospinal fluid lags behind blood glucose levels by 2–4 hours.

Fasting to 4 hours postpradially 50–80% of serum glucose.

Adult 40–80 mg/dL 2.2–4.4 mmol/L

Premature infant 24–63 mg/dL 1.3–3.5 mmol/L

Full-term infant 34–119 mg/dL 1.9–6.6 mmol/L

Child 35–75 mg/dL 1.9–4.1 mmol/L

Peritoneal fluid 70–100 mg/dL 3.8–5.5 mmol/L

Pleural fluid Same as blood glucose level, with a time lag

of 2–4 hours or no less than 40 mg/dL (2.2

mmol/L) below blood glucose

Fasting blood 60–110 mg/dL 3.3–6.1 mmol/L

Synovial fluid No more than 10 mg/dL (0.6 mmol/L SI

units) lower than good glucose level

Clinical Relevance

Persistent Hyperglycemia

¾ Diabetes mellitus

¾ Adrenal cortical hyperactivity (Cushing’s syndrome)

¾ Acromegaly

¾ Obesity.

Transient Hyperglycemia

¾ Pheochromocytoma

¾ Severe liver disease

¾ Acute stress reaction (physical or emotional)

¾ Shock

¾ Convulsions.

Persistent Hypoglycemia

¾ Insulinoma

¾ Adrenal cortical insufficiency

¾ Hypopituitarism

¾ Galactosemia

¾ Ectopic insulin production from tumors.

Transient Hypoglycemia

¾ Acute alcohol ingestion

¾ Drugs: Salicylates, anti-tuberculosis agents

¾ Severe liver disease

¾ Several glycogen storage diseases

¾ “Functional” hypoglycemia

¾ Hereditary fructose intolerance.

492 Concise Book of Medical Laboratory Technology: Methods and Interpretations URIC ACID

Uricase/PAP Method

(Courtesy: Tulip Group of Companies)

For the determination of uric acid in serum or plasma (for

in vitro diagnostic use only).