Studies have cited the presence of heterophile antibodies
during the course of infection with infectious mononucleosis.
Immutex is a ready-to-use, uniform suspension of
stabilized, specially treated horse erythrocytes highly
specific for heterophile antibodies associated with infectious
mononucleosis. The reagent does not react with normal
Each batch of reagent undergoes rigorous quality
control at various stages of manufacture for its specificity,
Store the reagents at 2 to 8°C. Do not freeze. The shelf-life
of the reagents is as per the expiry date mentioned on the
Immutex is a rapid slide hemagglutination test for the
detection of heterophile antibodies. Immutex IM reagent
will agglutinate when mixed with serum containing
heterophile antibodies. No agglutination indicates
absence of heterophile antibodies.
1. In vitro diagnostic reagent for laboratory and
professional use only. Not for medicinal use.
2. All the components derived from human source have
been tested for HBsAg and Anti-HIV antibodies and
found to be nonreactive. However, handle the material
3. The reagents contain thimerosal 0.01% as preservative.
Avoid contact with skin or mucosa. On disposal, flush
with large quantities of water.
4. The reagent can be damaged due to microbial
contamination or on exposure to extreme temperatures.
It is recommended that the performance of reagents
should be verified with known positive and negative
controls provided with the kit.
5. Ensure resuspension of the stabilized erythrocyte
reagent before use to improve test readability by gently
6. Do not interchange vial droppers.
7. Only a clean and dry glass slide must be used. Clean
the slide with distilled water and wipe dry.
Sample Collection and Preparation
No special preparation of the patient is necessary prior to
specimen collection by approved techniques. Though fresh
serum is preferable, serum specimens stored at 2 to 8°C for
up to 24 hours, can also be used in case of delay in testing.
Do not use hemolyzed or contaminated specimens. Turbid
specimens should be centrifuged or allowed to settle and
only the clear supernatant should be used for testing.
Materials Provided with the Kit
Immutex IM reagent, Positive Control, Negative Control.
Glass slide with six reaction circles, sample dispensing
pipettes, mixing sticks and rubber teats.
A high intensity direct light source, stopwatch.
Bring all reagents and samples to room temperature before
1. Gently mix the Immutex IM reagent to resuspend the
stabilized horse erythrocyte reagent.
2. Place one drop of the sample to be tested onto one of
the reaction circles of the glass slide using a sample
dispensing pipette provided with the kit.
3. Place one drop of the positive and negative control
onto separate reaction circles of the glass slide.
4. Add one drop of Immutex IM reagent to the test
5. Add one drop of Immutex IM reagent to each of the
6. Mix with separate mixing sticks, spreading the mixture
uniformly over the entire reaction circle.
7. Immediately start the stopwatch. Rock the slide gently,
back and forth, for 2 minutes.
8. Leave the slide undisturbed on the work table for a
9. Pick up the slide at the end of 1 minute and observe for
agglutination by rocking the slide gently back and forth.
Agglutination is a positive test result and indicates
presence of heterophile antibodies in the test specimen.
No agglutination is a negative test result and indicates
absence of heterophile antibodies in the test specimen.
1. Markedly lipemic, hemolyzed and contaminated
serum sample could produce nonspecific results.
2. Use of plasma rather than serum can lead to false
3. Heterophile antibodies may be found in disease
other than infectious mononucleosis. Low titers have
been detected in cytomegalic inclusion disease and
4. Do not read the results beyond indicated testing time
5. As with all diagnostic tests, the result of the test should
be correlated with clinical findings to arrive at the final
6. The reagent performance should be validated by
occasionally running the positive and negative
controls provided with the kit.
2. Markedly lipemic and contaminated serum
samples could produce non-specific results
Do not use lipemic and contaminated serum samples for testing
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